A Continuous Flow Microelectrophoretic Module for Protein Separation

Abstract

In this paper, we are presenting the development of a microfluidic electrically driven SPLITT (Split flow thin fractionation) device, which can be used for online protein separation. The microfluidic network is realized by means of a three times laminated structure (thermo-compression lamination) of a thin dry film, Ordyl SY 355, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.