Isolation of Extracellular Vesicles from Breast Milk

Abstract

Isolation of extracellular vesicles (EVs) from breast milk enables the downstream analysis of the contents, including various proteins, lipids, and RNAs (messenger RNAs and microRNAs). Here, we present the method of centrifugation with ExoQuick™ precipitation to isolate EVs from breast milk. It has been proven to be a rapid and effective alternative compared to conventional differential centrifugation method.