Photo-Induced Antifungal Activity of Chitosan Composite Film Solution with Nano-Titanium Dioxide and Nano-Silver

Abstract

This study was conducted to investigate the UV light-induced effect of chitosan-titanium dioxide-silver (CTS-TiO2-Ag) nanocomposite film solution against Penicillium steckii and the underlying physiological mechanism of this effect. With longer UV exposure time, pathogen inhibition increased. UV-photoinduced treatment for 120 min produced the smallest P. steckii colony diameter, at 4.85 mm. However, when this treatment was followed by 8 h of storage, the conductivity of the P. steckii culture medium reached its highest level, at 713 microsiemens per cm. After a 120-h growth period on mangoes under the same conditions, the lesion diameters and proportion of infected mangoes reached 12.61 mm and 41.67%, respectively. Because the P. steckii cell membrane was severely disrupted, its permeability increased, causing serious extravasation of intracellular protein and nucleic acid material. Malondialdehyde, catalase, and superoxide dismutase in the P. steckii cells reached maximum concentrations (2.1106 μmol/mL, 44.06 U/mL, and 24.67 U/mL, respectively) after 8 h of incubation. These results indicate significant P. steckii inhibition by the UV light induction of the CTS-TiO2-Ag nanocomposite film solution.