Jatropha multifida L stem sap gel versus Aloe vera gel to post-gingivectomy healing process

Abstract

Introduction: Gingivectomy is cutting the gingiva during a gingival enlargement condition that causes an open wound in the gingiva. Jatropha multifida L gel contains active ingredients of alkaloids, flavonoids, saponins and tannins, which are thought to accelerate the wound healing process, but its use in dentistry is limited. The objective of this research is to determine the effectiveness of the application of Jatropha multifida L stem sap gel in the wound healing process post-gingivectomy in Wistar rats (Rattus novergicus) compare with Aloe vera gel (Aloe Barbadensis Miller) by using parameters the number of fibroblasts, blood vessels and the density of collagen fibers. Methods: Rats were divided into 4 groups, namely group K (Aloe vera gel), P1 (Jatropha multifida L stem sap gel 2,5%), P2 (Jatropha multifida L stem sap gel 5%) and P3 (Jatropha multifida L stem sap gel 10%). Each group was given the gel twice a day in the wound area; then the wound was observed microscopically by Haematoxylin Eosin staining to count the number of fibroblasts and blood vessels also Masson Trichrome staining to observe the density of collagen fibers on day 3 and 7. Results: The data obtained were statistically analyzed using the One Way Anova, Dunnet and Kruskal-Wallis test. One Way Anova test shows there is a significant difference (p<0.05) in the number of fibroblasts, and the Dunnet test shows there is a significant difference (p<0.05) in the number of fibroblasts in Jatropha multifida L stem sap gel group 2.5% and 5 % on day 3. One Way Anova test shows no significant difference (p>0.05) in the number of blood vessels. The Kruskal-Wallis test shows no significant difference (p>0.05) in collagen fiber density. Conclusion: Jatropha multifida L Stem Sap gel has the same potential as Aloe vera gel in accelerating wound healing after gingivectomy in Wistar rats.