Comparison of Milk Ring Test; Serum Plate Agglutination Test and Polymerase Chain Reaction for the Detection of Bovine Brucellosis

Abstract

In the following study, comparative efficacy of Milk Ring Test (MRT), Serum Plate Agglutination Test (SPAT) was determined by calculating the sensitivities and specificities for detection of Brucellosis in bovines from district Peshawar of Khyber PakhtunKhwa, Pakistan. Considering Polymerase Chain Reaction (PCR) as a gold standard diagnostic test, MRT showed very low sensitivity (4.8%), while its specificity was 90.9 %, likewise, SPAT also showed low sensitivity (41 %), while its specificity was 66.7% in bovines. Statistically, high specificities and low sensitivities of MRT and SPAT in bovines recommended the poor efficacy of these tests, when used individually in contrast to PCR. As PCR is more reliable test, while considering its sensitivity for antigen detection as compare to antibody detection, therefore, PCR may be used as a routine screening test in clinical practices in farms animals for detection of Brucellosis. All copyrights reserved to Nexus® academic publishers