Angiogenesis, defi ned as the growth of new blood vessels from existing ones, plays a key role in development, growth, and tissue repair. Its necessary role in tumor growth and metastasis has led to the creation of a new category of anti-angiogenic cancer therapies. Preclinical development and evaluation of potential drug candidates require models that mimic real microvascular networks. Here, we describe the rat mesentery culture model as a simple ex vivo assay that offers time-lapse imaging of intact microvascular network remodeling and demonstrate its application for anti-angiogenic drug testing.
CITATION STYLE
Azimi, M. S., Lacey, M., Mondal, D., & Murfee, W. L. (2016). An ex vivo tissue culture model for anti-angiogenic drug testing. In Methods in Molecular Biology (Vol. 1464, pp. 85–95). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3999-2_8
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