Abstract
Methylation context sensitive enzyme ddRAD (MCSeEd) is a NGS-based method for genome-wide investigations of DNA methylation at different contexts requiring only low to moderate sequencing depth. It is particularly useful for identifying methylation changes in experimental systems challenged by biotic or abiotic stresses or at different developmental stages.
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Di Marsico, M., Cerruti, E., Comino, C., Porceddu, A., Acquadro, A., Capomaccio, S., … Albertini, E. (2020). MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation. In Methods in Molecular Biology (Vol. 2093, pp. 47–64). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0179-2_4
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