Chromatin immunoprecipitation (ChIP) is a powerful procedure to investigate the interactions between proteins and DNA. ChIP-chip combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in vivo. This genome-wide analysis of protein-DNA association is carried out in several steps including chemical cross-linking, cell lysis, DNA fragmentation and immunoaffinity purification that allow the identification of DNA interactions and provide a powerful tool for genome-wide investigations. Immunoprecipitated DNA fragments associated with the desired protein are amplified, labelled and hybridized to DNA microarrays to detect enriched signals compared to a labelled reference sample.
CITATION STYLE
Durand-Dubief, M., & Ekwall, K. (2009). Chromatin immunoprecipitation using microarrays. Methods in Molecular Biology (Clifton, N.J.), 529, 279–295. https://doi.org/10.1007/978-1-59745-538-1_18
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