A simple and rapid method for Shiga toxin purification based on specific binding to the Galα1 → 4Galβ1 → 4Glc globotrioside trisaccharide covalently linked to polyacryl/polyvinyl (Fractogel) has been developed. A cell-free sonicate-filtrate of Shigella dysenteriae type 1, strain 114Sd was passed over the globotrioside-Fractogel column, and bound toxin eluted with 6 M guanidine-HCl. A yield of 36 mg pure toxin/1 sonicate-filtrate was otained, i.e. a one step 1224-fold purification. The recovery of biologically active toxin was 87%. The toxin was purified to homogeneity as shown by native PAGE and SDS-PAGE. © 1989.
Ryd, M., Alfredsson, H., Blomberg, L., Andersson, Å., & A. Lindberg, A. (1989). Purification of Shiga toxin by α-d-galactose-(1 → 4)-β-d-galactose-(1 → 4)- β-d-glucose-(1 →) receptor ligand-based chromatography. FEBS Letters, 258(2), 320–322. https://doi.org/10.1016/0014-5793(89)81684-9