Recently, phytochrome has been shown to regulate not only transcription but also alternative splicing at a similar genomic scale in Arabidopsis. Here I describe the protocols for detecting phytochrome-mediated light-responsive alterations in alternative splicing in Arabidopsis, using RT-PCR technique followed by quantification with the Bioanalyzer.
CITATION STYLE
Matsushita, T. (2019). Regulation of Alternative Splicing by Phytochrome. In Methods in Molecular Biology (Vol. 2026, pp. 143–148). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9612-4_11
Mendeley helps you to discover research relevant for your work.