Metabolomics aims at the identification and quantification of key-end point metabolites, basically polar, in order to study changes in biochemical activities in response to pathophysiological stimuli or genetic modifications. Targeted profiling assays have enjoyed a growing popularity during the last years with LC-MS/MS as a powerful tool for development of such (semi-) quantitative methods for a large number of metabolites. Here we describe a method for absolute quantification of ca. 100 metabolites belonging to key metabolite classes such as sugars, amino acids, nucleotides, organic acids, and amines with a hydrophilic interaction liquid chromatography (HILIC) system comprised of ultra (high) performance liquid chromatography (UHPLC) with detection on a triple-quadrupole mass spectrometer operating in both positive and negative electrospray ionization modes.
CITATION STYLE
Virgiliou, C., Gika, H. G., & Theodoridis, G. A. (2018). HILIC-MS/MS multi-targeted method for metabolomics applications. In Methods in Molecular Biology (Vol. 1738, pp. 65–81). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7643-0_5
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