The use of in vitro assays, such as glutathione S-transferase (GST) pull-downs, enables the study of complex cellular processes in a simplified form. Pull-down assays facilitate the discovery and detailed study of protein– protein interactions, which can then be extrapolated to the cellular environment. Here, we describe the expression, purification and use of a multi-SUMO platform to identify SUMO-interacting proteins. This SUMO-platform can be easily expressed and purified from bacterial cells for use as baits in pull-down assays. This methodology facilitates the discovery of novel SUMO-binding proteins or further characterization of SUMO with known binding partners.
CITATION STYLE
Aguilar-Martínez, E., & Sharrocks, A. D. (2016). The use of multimeric protein scaffolds for identifying multi-SUMO binding proteins. In Methods in Molecular Biology (Vol. 1475, pp. 195–204). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6358-4_14
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