Tumor Purity in Preclinical Mouse Tumor Models

Abstract

Tumor biology is determined not only by immortal cancer cells but also purity also turns out to be an intrinsic property with model and cancer by the tumor microenvironment consisting of noncancerous cells and ex- specificities. Computational and pathology analysis confirmed the impact tracellular matrix, together they dictate the pathogenesis and response to on tumor purity by the diverse stromal and immune profiles. Our study treatments. Tumor purity is the proportion of cancer cells in a tumor. It deepens the understanding of mouse tumor models, which will enable their is a fundamental property of cancer and is associated with many clinical better and novel uses in developing cancer therapeutics, especially ones features and outcomes. Here we report the first systematic study of tumor targeting tumor microenvironment. purity in patient-derived xenograft (PDX) and syngeneic tumor models us- Significance:PDX models are an ideal experimental system to study tumor ing next-generation sequencing data from >9,000 tumors. We found that purity because of its distinct separation of human tumor cells and mouse tumor purity in PDX models is cancer specific and mimics patient tumors, stromal and immune cells. This study provides a comprehensive view of tuwith variation in stromal content and immune infiltration influenced by mor purity in 27 cancers in PDX models. It also investigates tumor purity in immune systems of host mice. After the initial engraftment, human stroma 19 syngeneic models based on unambiguously identified somatic mutations. in a PDX tumor is quickly replaced by mouse stroma, and tumor purity It will facilitate tumor microenvironment research and drug development then stays stable in subsequent transplantations and increases only slightly in mouse tumor models. by passage. Similarly, in syngeneic mouse cancer cell line models, tumor