The Coexistence of Extended-Spectrum β-lactamase and Metallo-β-Lactamase Genes in Gram-Negative Bacteria

Abstract

The coexistence of different classes of beta-lactamases in multiple drug-resistant (MDR) bacteria has widely increased, thus rising diagnostic and treatment challenges. The study aimed at evaluating the prevalence of extended-spectrum beta-lactamase (ESBLs) and Metallo-β-lactamase (MBL) genes in confirmed ESBL producing Gram-negative (G-) bacteria. A stock of 220 G- isolates were studied for producing ESBL and MBL using phenotypic and PCR methods. The results showed that ESBLs were identifi