Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
CITATION STYLE
Reese, C. B., & Song, Q. (1997). Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates. Nucleic Acids Research, 25(14), 2943–2944. https://doi.org/10.1093/nar/25.14.2943
Mendeley helps you to discover research relevant for your work.