Using engineered nucleases, such as Zinc Finger Nucleases (ZFNs) or Transcription Activator-Like Effector Nucleases (TALENs), to make targeted genomic modifications has become a common technique to create new model organisms and custom cell lines, and has shown great promise for disease treatment. However, these nucleases have the potential for off-target cleavage that could confound interpretation of experimental results and be detrimental for therapeutic use. Here, we describe a method to test for nuclease cleavage at potential off-target sites predicted by bioinformatics models. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Fine, E. J., Cradick, T. J., & Bao, G. (2014). Identification of off-target cleavage sites of zinc finger nucleases and TAL effector nucleases using predictive models. Methods in Molecular Biology, 1114, 371–383. https://doi.org/10.1007/978-1-62703-761-7_24
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