Abstract
The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole- virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus- negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.
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CITATION STYLE
Valassina, M., Soldateschi, D., Dal Maso, G. M., Santini, L., Bianchi, S., Valensin, P. E., & Cusi, M. G. (1998). Diagnostic potential of Toscana virus N protein expressed in Escherichia coli. Journal of Clinical Microbiology, 36(11), 3170–3172. https://doi.org/10.1128/jcm.36.11.3170-3172.1998
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