Efecto del extracto etanólico Erythroxylum novogranatense var. truxillense y Erythroxylum coca var. coca frente al Streptococcus mutans.

  • Salcedo Calderón M
  • Moromi Nakata H
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Abstract

Objetivos: Comparar el efecto antibacteriano in vitro de los extractos etanólico de dos variedades de hoja de coca: Erythroxylum coca var. coca y Erythroxylum novogranatense var. truxillense sobre el Streptococcus mutans. Material y métodos: Mediante el método de difusión por discos, se procedió a la aplicación de los extractos etanólicos de las dos variedades a las concentraciones 100%, 50%, 25% y 12,5% con controles positivo de Clorhexidina 0,12 % y negativo de Alcohol 96º en los cultivos de la cepa de Streptococcus mutans (ATCC 25175) sembrados en Agar Tripticasa Soya. La incubación se realizó a 37 °C por 48 horas en condiciones de anaerobiosis parcial. El análisis estadístico se realizó con el programa SPSS Versión 22. Mediante la Prueba U de Mann Whitney y Kruskal Wallis. Resultados: El extracto etanólico de Erythroxylum coca var. coca al 100% y 50% presentaron mayor halo de inhibición 18,65± 2.434 y 17,10 ± 2,654 con respecto al extracto etanólico de Erythroxylum novogranatense var. truxillense que fueron de 15,30 ± 1.895 y 14,05 ± 1.932. respectivamente. Conclusiones: Se determinó que el extracto etanólico de Erythroxylum coca var. coca al 100% y 50% tiene mayor efecto antibacteriano que la variedad Erythroxylum novogranatense var. truxillense al 100% frente al Streptococcus mutans.Objectives: To compare the antibacterial effect of the ethanolic extracts of two varieties of coca leaf “in vitro”: Erythroxylum coca var. coca and Erythroxylum novogranatense var. truxillense on Streptococcus mutans. Material and methods: Using the method of diffusion by discs, the ethanolic extracts of the two varieties were applied at concentrations 100%, 50%, 25% and 12.5% with positive controls of Chlorhexidine 0.12% and negative of 96º Alcohol in the cultures of the Streptococcus mutans strain (ATCC 25175) sown in Tripticasa Soya Agar. Incubation was performed at 37 ° C for 48 hours under conditions of partial anaerobiosis. The statistical analysis was carried out with the SPSS Version 22 program by means of the Mann Whitney U Test and Kruskal Wallis. Results: Erythroxylum coca var. coca 100% and 50% had a greater inhibition halo 18.65 ± 2.434 and 17.10 ± 2.654 with respect to the ethanolic extract of Erythroxylum novogranatense var. truxillense that were 15.30 ± 1.895 and 14.05 ±1.932. respectively. Conclusions: It was determined that the ethanolic extract of Erythroxylum coca var. coca 100% and 50% has a greater antibacterial effect than the Erythroxylum novogranatense var. truxillense 100% against Streptococcus mutans.

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Salcedo Calderón, M. D. R., & Moromi Nakata, H. (2022). Efecto del extracto etanólico Erythroxylum novogranatense var. truxillense y Erythroxylum coca var. coca frente al Streptococcus mutans. Revista Estomatológica Herediana, 32(4), 365–370. https://doi.org/10.20453/reh.v32i4.4376

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