HPTLC analysis, antioxidant and antidiabetic activities of ethanol extract of moss fissidens grandiflora

Abstract

The phytochemical composition, antioxidant and antidiabetic profile of ethanol extract of Fissidens grandiflora was investigated for the first time. The phytochemical analysis of Fissidens grandiflora confirmed the presence of flavonoids, phenolics, steroids and tannins. Additionally, Fissidens grandiflora contained a high amount of total flavonoids of 101.40±0.38 mg Q/g. The high-performance thin-layer chromatography analysis of Fissidens grandiflora identified 3 unknown components with different Rf values and area percentage. Fissidens grandiflora showed better inhibition of superoxide free radicals, 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radicals and ferric ions with IC50 values of 81.0, 85.0 and 92.6 µg/ml, respectively. Additionally, Fissidens grandiflora depicted a prominent inhibitory profile against α-glucosidase and pancreatic α-amylase with IC50 values of 150.0 and 200.0 µg/ml, respectively. To conclude, the proposed mechanism of action of moss Fissidens grandiflora could be by inhibiting free radicals and particularly digestive enzymes.