Electrochemical analysis of indigo carmine using polyarginine modified carbon paste electrode

Abstract

Suitable electrocatalytic method is established for the selective determination of indigo carmine (IC) at polyarginine modified carbon paste electrode (PAMCPE). Surface morphological study of bare carbon paste electrode (CPE) and PAMPCE is done by field emission scanning electron microscopy (FESEM). The influence of different parameters such as IC concentration, solution pH and potential scan rate on the electrode responses is studied using cyclic and differential pulse voltammetry techniques. The prepared PAMCPE shows better electrochemical response towards IC than CPE. No interference is noticed at simultaneous presence of IC and riboflavin (RF) in the solution. The electrocatalytic current of IC at PAMPCE is varied linearly with its concentration in two separate ranges, from 2×10-7 to 10-6 M, and 1.5×10-6 to 3.5×10-6 M. Limits of detection (LOD) and quantification (LOQ) are determined as 2.53×10-8 and 8.43×10-8 M, respectively. The developed PAMCPE is showing successful reproducibility and stability. It is also found sensitive and reliable for trace amounts of IC in some real water and food samples. Since preparation of PAMCPE sensor is simple and easy, it could become a part of the standard method for determination of IC in real samples.