Nowadays polysaccharide chiral stationary phases are very popular and this originates for many reasons: (1) their wide applications window, (2) numerous different chemistry availability either in coated or in immobilized versions, and (3) large loading capability useful for preparative scale. Indeed chiral separations remain a hot topic (particularly in the pharmaceutical market) and in this field supercritical fluid chromatography is emerging rapidly. However, its use is more complex than high-performance liquid chromatography. The presented example illustrates analytical scale chiral separation method development and the effect of each operating parameter, i.e., flow rate, outlet pressure, and temperature variations on a chiral separation.
CITATION STYLE
Lipka, E. (2019). Applications of chiral supercritical fluid chromatography. In Methods in Molecular Biology (Vol. 1985, pp. 303–319). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9438-0_16
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