Hexokinase activity as marker to assess time of harvest of foot‐and‐mouth disease virus in BHK21 Cl13 cell cultures

Abstract

Hexokinase (B.C. 2.7.1.1) activity as a marker enzyme during FMD viral infection has been observed spectrophotometrically in a system coupled with glucose‐6‐phosphate dehydrogenase, in supernatants of BHK21Cl13 suspension as well as anchored cell culture at a minimum of 104 infective virus particles/ml. Specific activity increased with virus concentration in culture supernatants and abruptly decreased with a fall in virus titer, as has been noted by TCID/50,146 S concentration, and enzyme‐linked immunosorbent assay (ELISA) readings. Copyright © 1986 John Wiley & Sons, Inc.