High performance liquid chromatographic determination of malondialdehyde in vegetable oils.

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Abstract

A simple high performance liquid chromatographic (HPLC) method has been developed for determining malondialdehyde (MDA) in vegetable oils. MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1-dansyl-pyrazole, was determined by HPLC, using a Zorbax sil column with mixed mobile phase of n-hexane-methylene chloride. MDA can be determined as 1-dansyl-pyrazole by fluorometric detection at a level of 0.01 ppm in vegetable oils.

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Hirayama, T., Yamada, N., Nohara, M., & Fukui, S. (1983). High performance liquid chromatographic determination of malondialdehyde in vegetable oils. Journal - Association of Official Analytical Chemists, 66(2), 304–308. https://doi.org/10.1093/jaoac/66.2.304

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