Gene expression patterns change dramatically in aging and age-related events. The DNA microarray is now recognized as a useful device in molecular biology and widely used to identify the molecular mechanisms of aging and the biological effects of drugs for therapeutic purpose in age-related diseases. Recently, numerous technological advantages have led to the evolution of DNA microarrays and microarray-based techniques, revealing the genomic modification and all transcriptional activity. Here, we show the step-by-step methods currently used in our lab to handling the oligonucleotide microarray and miRNA microarray. Moreover, we introduce the protocols of ribonucleoprotein [RNP] immunoprecipitation followed by microarray analysis (RIP-chip) which reveal the target mRNA of age-related RNA-binding proteins. © Springer Science+Business Media, New York 2013.
CITATION STYLE
Masuda, K., Kuwano, Y., Nishida, K., & Rokutan, K. (2013). Application of DNA microarray technology to gerontological studies. Methods in Molecular Biology, 1048, 285–308. https://doi.org/10.1007/978-1-62703-556-9_19
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