Gene modification of T cells with chimeric T-cell receptor (TCR) transgenes offers a novel way to generate tumor-specific T cells for cancer immunotherapy. Retroviruses have been utilized as the most common means of efficiently transducing primary T lymphocytes with these transgenes. In this section we describe methods for generation of chimeric TCR's and utilization of retroviral vectors for efficient transduction of these transgenes in primary mouse T lymphocytes.
CITATION STYLE
Howland, L. J., Haynes, N. M., & Darcy, P. K. (2010). Generation of chimeric T-cell receptor transgenes and their efficient transfer in primary mouse T lymphocytes. Methods in Molecular Biology (Clifton, N.J.), 651, 291–306. https://doi.org/10.1007/978-1-60761-786-0_17
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