Pengaruh Iradiasi Sinar Gamma pada Pertumbuhan Kalus dan Keragaman Planlet Tanaman Nilam

Abstract

The Effect of Gamma Irradiation on Calli Growth and Patchouly Planlet Variation. Abdul Kadir, Surjono H. Sutjahjo, Gustav A. Wattimena, and Ika Mariska. This research was objected to study the effect of gamma irradiation on growth of calli and plantlet and phenotypic variation of patchouly plantlet. Research was conducted at the Tissue Culture Laboratory of Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor. Gamma irradiation treatment was done at the Centre for Research and Development of Isotop and Radiation Technology, BATAN, Jakarta. The treatment consisted of 5 level of irradiation i.e. 0 (control), 5, 10, 15, and 20 Gy of gamma irradiation. The result showed that gamma irradiation of 20 Gy decrease calli quality index and increased percentage of calli death and inhibited calli growth at 30, 60 and 90 days after irradiation, also decrese number of shoots. Gamma irradiation of 5 Gy and 10 Gy increased growth planlet compared 15 Gy and 20 Gy, meanwhile gamma irradiation at 20 Gy induced high frequency of phenotypic variation of patchouly plantlet.