A three-dimensional (3D) tissue generated in vitro is a promising source to study developmental biology and regenerative medicine. In the last decade, Yoshiki Sasai’s group have developed a 3D stem cell culture technique known as SFEBq and demonstrated that embryonic stem cells (ESCs) have an ability to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs.
CITATION STYLE
Kuwahara, A., Nakano, T., & Eiraku, M. (2017). Generation of a three-dimensional retinal tissue from self-organizing human ESC culture. In Methods in Molecular Biology (Vol. 1597, pp. 17–29). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6949-4_2
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