Hypocrea stromatica sp. nov. teleomorfo de Trichoderma stromaticum

  • Bezerra J
  • Costa J
  • Bastos C
  • et al.
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Abstract

Trichoderma stromaticum é um fungo antagonista a Crinipellis perniciosa, agente causal da vassoura-de-bruxa do cacaueiro (Theobroma cacao). Estromas periteciais de Hypocrea sp. formaram-se sobre a superfície de frutos mumificados e vassouras secas de cacaueiros em áreas onde T. stromaticum tinha sido pulverizado, experimentalmente, no controle biológico de C. perniciosa. Ascósporos obtidos dos estromas de Hypocrea sp. originaram colônias idênticas às de T. stromaticum. DNA genômico de colônias provenientes de ascósporos desta Hypocrea sp., de conídios de T. stromaticum e de Fusarium sp. (controle negativo) foi extraído e amplificado com oito "primers" decâmeros para obtenção de marcadores RAPD. Observaram-se similaridades genéticas de 0,82 a 0,96 entre os isolados ascospóricos e conidiais com base nos marcadores RAPD e de 0,06 a 0,09 entre estes e o isolado de Fusarium sp. O teleomorfo de T. stromaticum é denominado H. stromatica Bezerra, Costa & Bastos sp. nov.Trichoderma stromaticum is a fungal antagonist to Crinipellis perniciosa, causal agent of cacao (Theobroma cacao)witches' broom disease. Peritecial stromata of a Hypocrea species developed on the surface of mummified pods and dried brooms of cacao, in areas where T. stromaticum had been applied, experimentally, as a biological agent to control C. perniciosa. Ascospores obtained from this Hypocrea sp. gave rise to colonies identical to those of T. stromaticum. Genomic DNA from cultures developed from ascospores of this Hypocrea sp., conidia of T. stromaticum and Fusarium sp. (negative control) was extracted and amplified with eight decamer primers to obtain RAPD markers. Genetic similarities from 0.82 to 0.96 were observed between ascosporic and conidial isolates based on RAPD markers, and from 0.06 to 0.09 between these and the Fusarim sp. isolate. The teleomorph of T. stromaticum is, thus, proposed as H. stromatica Bezerra, Costa & Bastos sp. nov.

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Bezerra, J. L., Costa, J. C. B., Bastos, C. N., & Faleiro, F. G. (2003). Hypocrea stromatica sp. nov. teleomorfo de Trichoderma stromaticum. Fitopatologia Brasileira, 28(4), 408–412. https://doi.org/10.1590/s0100-41582003000400010

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