Telomerase activity in pancreatic juice for the preoperative diagnosis of pancreatic cancer

Abstract

Although many diagnostic challenges have been tried in pancreatic cancer, its prognosis still remains poor. One of the reason that it is difficult to diagnose using present diagnostic procedure in early stage of pancreatic cancer. Therefore, we tried new procedure using telomerase activity from pancreatic juice. In this study, telomerase was assayed by use of pancreatic duct cells obtained preoperatively from 32 cases with pancreatic duct abnormalities. Telomerase activity was undetectable in all 17 subjects with benign pancreatic diseases, whereas 13 (87%) of 15 subjects with pancreatic cancer had detectable telomerase activity. Interestingly, we also detected telomerase activity in the case of carcinoma in situ. If future studies confirm, the detection of telomerase activity of pancreatic duct cells may become an useful tool for diagnosis of pancreatic cancer, perhaps at early stages.