Streptomyces tirandamycinicus sp. Nov., a novel marine sponge-derived actinobacterium with antibacterial potential against streptococcus agalactiae

Abstract

A novel actinobacterium, strain HNM0039T, was isolated from a marine sponge sample collected at the coast of Wenchang, Hainan, China and its polyphasic taxonomy was studied. The isolate had morphological and chemical characteristics consistent with the genus Streptomyces. Based on the 16S rRNA gene sequence analysis, strain HNM0039Twas closely related to Streptomyces wuyuanensis CGMCC 4.7042T(99.38%) and Streptomyces spongiicola HNM0071T(99.05%). The organism formed a well-delineated subclade with S. wuyuanensis CGMCC 4.7042Tand S. spongiicola HNM0071Tin the Streptomyces 16S rRNA gene tree. Multi-locus sequence analysis (MLSA) based on five housekeeping gene alleles (atpD, gyrB, rpoB, recA, trpB) further confirmed their relationship. DNA–DNA relatedness between strain HNM0039Tand its closest type strains, namely S. wuyuanensis CGMCC 4.7042Tand S. spongiicola HNM0071T, were 46.5 and 45.1%, respectively. The average nucleotide identity (ANI) between strain HNM0039Tand its two neighbor strains were 89.65 and 91.44%, respectively. The complete genome size of strain HNM0039Twas 7.2 Mbp, comprising 6226 predicted genes with DNA G+C content of 72.46 mol%. Thirty-one putative secondary metabolite biosynthetic gene clusters were also predicted in the genome of strain HNM0039T. Among them, the tirandamycin biosynthetic gene cluster has been characterized completely. The crude extract of strain HNM0039Texhibited potent antibacterial activity against Streptococcus agalactiae in Nile tilapia. And tirandamycins A and B were further identified as the active components with MIC values of 2.52 and 2.55 μg/ml, respectively. Based on genotypic and phenotypic characteristics, it is concluded that strain HNM0039Trepresents a novel species of the genus Streptomyces whose name was proposed as Streptomyces tirandamycinicus sp. nov. The type strain is HNM0039T(= CCTCC AA 2018045T= KCTC 49236T).