Macrophages are cells of the immune system that have been suggested as important regulators of whole-body metabolism in mammals. In obesity, adipose tissue macrophages (ATMs) are thought to play both a detrimental and a beneficial role in the regulation of insulin sensitivity. Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in ATMs in obese mice. Using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by ATMs in the regulation of metabolism.
CITATION STYLE
Barreby, E., Sulen, A., & Aouadi, M. (2019). Glucan-encapsulated siRNA particles (GeRPs) for specific gene silencing in adipose tissue macrophages. In Methods in Molecular Biology (Vol. 1951, pp. 49–57). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9130-3_4
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