Mapping of DNA methylation is essential in understanding the process of HBV covalently closed circular DNA (cccDNA) transcription. Here, bisulfite sequencing PCR and methylation-specific PCR, two PCR-based approaches used in determining and quantifying the DNA methylation pattern, are described.
CITATION STYLE
Zhang, Y., Mao, R., Guo, H., & Zhang, J. (2017). Detection of HBV cccDNA methylation from clinical samples by bisulfite sequencing and methylation- specific PCR. In Methods in Molecular Biology (Vol. 1540, pp. 73–84). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6700-1_7
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