Identification of microsatellite markers in the rye genome

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Abstract

The rye genomic library, which consists of DNA fragments in the range of 0.5-1.1 kb, was screened for the presence of tri- and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R. © 2006 by the University of Wrocław.

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Bolibok, H., Rakoczy-Trojanowska, M., Wyrzykowska, M., Radecka, M., & Orczyk, W. (2006). Identification of microsatellite markers in the rye genome. Cellular and Molecular Biology Letters, 11(2), 291–298. https://doi.org/10.2478/s11658-006-0023-5

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