Induction of Tomatine in Tomato Plant by an Avirulent Strain of Pseudomonas solanacearum.

Abstract

Tomato plants inoculated with an avirulent strain of Pseudomonas solanacearum a growth substance level than those at a higher inhibitory Str-10 op type) produced (strain was identified control but not in stem. The substance to be in root system of uninoculated component and chemical TLC, UV spectrometry tomatine by Mass Spectrometry, contents were 113 and 152ƒÊg/g The tomatine fresh five days after inoculation analysis. root when op type was inoculated Str-10 respectively. Tomatine content at the inoculum in the uninoculated concentrations of 108 and 109cfu/ml, plants was 65.9ƒÊg/g. control Tomatine content nine days after inoculation reached 450ƒÊg/g. In an in vitro experiment, 100ƒÊg/ disk tomatine of authentic for growth sufficient inhibition and approximately of P. solanacearum. tomatine were 150ƒÊg/disk of extracted and authentic Both extracted tomatine exhibited bacteriostatic activity against P. solanacearum. Treatment with heat-killed cells or culture filtrate of Str-10 op type failed to increase tomatine content in the root tissue.