A 160-bp palindrome is a rad50·rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe

58Citations
Citations of this article
17Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Palindromic sequences can form hairpin and cruciform structures that pose a threat to genome integrity. We found that a 160-bp palindrome (an inverted repeat of 80 bp) conferred a mitotic recombination hotspot relative to a control nonpalindromic sequence when inserted into the ade6 gene of Schizosaccharomyces pombe. The hotspot activity of the palindrome, but not the basal level of recombination, was abolished by a rad50 deletion, by a rad50S "separation of function" mutation, or by a rad32-D25A mutation in the nuclease domain of the Rad32 protein, an Mre11 homolog. We propose that upon extrusion of the palindrome the Rad50·Rad32 nuclease complex recognizes and cleaves the secondary structure thus formed and generates a recombinogenic break in the DNA.

References Powered by Scopus

The double-strand-break repair model for recombination

1903Citations
N/AReaders
Get full text

The distribution of the numbers of mutants in bacterial populations

1157Citations
N/AReaders
Get full text

Structural biology of Rad50 ATPase: ATP-driven conformational control in DNA double-strand break repair and the ABC-ATPase superfamily

835Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair

853Citations
N/AReaders
Get full text

Comparative genomics and molecular dynamics of DNA repeats in eukaryotes

422Citations
N/AReaders
Get full text

ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1

356Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Farah, J. A., Hartsuiker, E., Mizuno, K. I., Ohta, K., & Smith, G. R. (2002). A 160-bp palindrome is a rad50·rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe. Genetics, 161(1), 461–468. https://doi.org/10.1093/genetics/161.1.461

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

36%

Researcher 4

36%

Lecturer / Post doc 2

18%

Professor / Associate Prof. 1

9%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 5

45%

Biochemistry, Genetics and Molecular Bi... 5

45%

Social Sciences 1

9%

Save time finding and organizing research with Mendeley

Sign up for free