The potential of carrot extract as a sunscreen to prevent apoptosis in white mice (Mus musculus) fibroblast cell cultures exposed to UVB light

Abstract

Introduction: UVB rays are one of the main external factors that play a role in the aging process. Apoptosis of fibroblasts affects skin aging, and the decline in fibroblasts is also caused by the reduced number and ability of growth factors and other hormones. Sunscreen has been used for a long time to protect the skin from the harmful effects of solar radiation, especially UVB rays. Antioxidant activity such as β-carotene and vitamin E from carrots can counteract free radicals and oxidative stress caused by UV radiation. This study aims to test whether in vitro administration of carrot extract can be used as a sunscreen to prevent apoptosis in mouse fibroblast cell cultures exposed to UVB light. Method: This research is a laboratory experimental study, using a Randomized Post-test Only Control Group Design. The research sample was fibroblast cell culture derived from the back skin of white mice (Mus Musculus) isolated with Collagenase 1 (primary culture). Observation of fibroblast apoptosis using propidium iodide staining. Data were analyzed using Shapiro-Wilk test, Levene’s test, One Way Anova and continued with LSD test. The confidence level in this study is 95%. Result: There was a significant difference between the control group and the carrot extract treatment group, but no significant difference was found between the SPF15 group and the carrot group (p>0.01). Conclusion: Carrot extract can provide a protective effect on the skin (fibroblasts) and prevent apoptosis.