The inflammatory response of the pulp after direct capping with platelet-rich plasma and enamel matrix derivative: A controlled animal study

Abstract

Aims: To evaluate the inflammatory response of the exposed pulp of incisor teeth in rats after direct pulp capping, using platelet rich plasma (PRP), enamel matrix derivate (EMD), mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH) 2). Methods: The study was conducted on 36 Wistar albino rats with a total of 144 incisor teeth. The pulps of 96 teeth of the rats were perforated and capped with different agents. Serving as the positive control group, the pulps of 24 teeth were only perforated and capped without capping agents, whereas the pulps of 24 teeth were us-ed as the negative control group without being perfo-rated (without any process). The research was ended with the extracting of the teeth on the 7th day -28th day. The teeth were taken to the routine and histolo-gical follows; cross sections were prepared and pain-ted with hematoxylen {&} eosin. All of the sections were evaluated in terms of inflammatory reaction by his-tologic analysis taken by light microscope. Statistical analysis was used. The normal distribution of all data was tested with the Mann Whitney U and the differ-ences between the groups were analyze dusing Kru-skal Wallis test at 0.05 level. Results: There are no statistically significant differences in terms of inflam-mation type and necrosis among the treatment groups on 7 days' post capping. However, improved inflam-matory cell accumulation, hyperemia and lowest ne-crosis were observed from the samples treated with PRP (p {