Susceptibility of mouse splenic cells to oxidative DNA damage by x-ray irradiation

Abstract

Susceptibility to oxidative stress by X-ray irradiation was examined in splenic cells of BDF1 mouse and fetal human lung fibroblasts, TIG-7. Survival rates of splenic cells irradiated with X-rays were lower than those of TIG-7 cells irradiated similarly. The content of 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxodG) immediately after X-ray irradiation in the DNA of splenic cells increased until 2 Gy irradiation, but remained constant above 2Gy. The 8-oxodG contents rose in proportion to the dose of X-rays in TIG-7 cells. Although the survival rate of splenic cells exposed to 1 Gy irradiation decreased with time, the survival rate of TIG-7 cells remained unchanged. The 8-oxodG content in splenic cells irradiated with X-rays did not decrease even 48 h after irradiation, while that in TIG-7 cells decreased with time, and recovered to the pre-irradiation level after 48 h. A DNA ladder was observed in splenic cells 2 h after X-ray irradiation, but the ladder was not found in fibroblasts. Furthermore, caspase-3 activity increased after X-ray irradiation of splenic cells. These results indicate that splenic cells are sensitive to oxidative stress induced by X-ray irradiation and that splenic cells damaged by even low doses of X-rays are removed through apoptosis rather than by a repair pathway. © 2004 Pharmaceutical Society of Japan.