HIV-1 Tat Regulates Occludin and A β Transfer Receptor Expression in Brain Endothelial Cells via Rho/ROCK Signaling Pathway

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Abstract

HIV-1 transactivator protein (Tat) has been shown to play an important role in HIV-Associated neurocognitive disorders. The aim of the present study was to evaluate the relationship between occludin and amyloid-beta (Aβ) transfer receptors in human cerebral microvascular endothelial cells (hCMEC/D3) in the context of HIV-1-related pathology. The protein expressions of occludin, receptor for advanced glycation end products (RAGE), and low-density lipoprotein receptor-related protein 1 (LRP1) in hCMEC/D3 cells were examined using western blotting and immunofluorescent staining. The mRNA levels of occludin, RAGE, and LRP1 were measured using quantitative real-Time polymerase chain reaction. HIV-1 Tat at 1 μg/mL and the Rho inhibitor hydroxyfasudil (HF) at 30 μmol/L, with 24 h exposure, had no significant effect on hCMEC/D3 cell viability. Treatment with HIV-1 Tat protein decreased mRNA and protein levels of occludin and LRP1 and upregulated the expression of RAGE; however, these effects were attenuated by HF. These data suggest that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-mediated changes in occludin, RAGE, and LRP1 in hCMEC/D3 cells. HF may have a beneficial influence by protecting the integrity of the blood-brain barrier and the expression of Aβ transfer receptors.

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Chen, Y., Huang, W., Jiang, W., Wu, X., Ye, B., & Zhou, X. (2016). HIV-1 Tat Regulates Occludin and A β Transfer Receptor Expression in Brain Endothelial Cells via Rho/ROCK Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2016. https://doi.org/10.1155/2016/4196572

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