Introduction: This work shows that 3% (v/v) human urine (HU) in semisolid Liver Infusion Tryptose (SSL) medium favors the growth of Trypanosoma cruzi and T. rangeli. Methods: Parasites were plated as individual or mixed strains on SSL medium and on SSL medium with 3% human urine (SSL-HU). Isolate DNA was analyzed using polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE). Results: SSL-HU medium improved clone isolation. PCR revealed that T. cruzi strains predominate on mixed-strain plates. PFGE confirmed that isolated parasites share the same molecular karyotype as parental cell lines. Conclusions: SSL-HU medium constitutes a novel tool for obtaining T. cruzi and T. rangeli clonal lineages.
CITATION STYLE
Fajardo, E. F., Cabrine-Santos, M., Ferreira, K. A. M., Lages-Silva, E., Ramírez, L. E., & Pedrosa, A. L. (2016). Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages. Revista Da Sociedade Brasileira de Medicina Tropical, 49(3), 369–372. https://doi.org/10.1590/0037-8682-0190-2015
Mendeley helps you to discover research relevant for your work.