There is a considerable interest in determining the role of individual oomycete effectors in promoting disease. Widely used strategies are based on manipulating effector-expression levels in the pathogen and by over-expressing particular effectors in the host by genetic transformation. In the case of the oomycete, Hyaloperonospora arabidopsidis (Hpa) genetic manipulation is not yet possible, so over-expression of predicted effectors in stably transformed Arabidopsis lines is used to investigate their capability for promoting virulence. Here, we describe a technique for quantifying pathogen growth based on the counting of asexual reproductive structures called sporangiophores in the compatible interaction between the Hpa isolate Noksl and the Col-0 Arabidopsis accession.
CITATION STYLE
Tomé, D. F. A., Steinbrenner, J., & Beynon, J. L. (2014). A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana. Methods in Molecular Biology, 1127, 145–158. https://doi.org/10.1007/978-1-62703-986-4_12
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