Assay Development and Measurement of the Aging Biomarker Humanin

Abstract

Biomarkers that reflect aging could be used to target age-related diseases with precision and monitor treatment efficacy. One such biomarker is humanin, a 24-amino acid mitochondrial-derived peptide encoded within the mitochondrial 16S rRNA gene. Humanin is measured in biological fluids, associates with many aging phenotypes, and attenuates aging in several animal models. In this chapter, we highlight the development and protocol of an enzyme-linked immunosorbent assay that quantifies humanin levels in biological fluid.