DEVELOPMENT OF NON-INVASIVE METHOD FOR BLOOD MICROCIRCULATION DISORDERS DIAGNOSTICS IN DIABETIC PATIENTS USING LASER DOPPLER FLOWMETRY

Abstract

Aim: To provide diagnostics of microcirculatory disorders with diabetes mellitus (DM) by using LDF. Materials and methods: On the first step we examined the condition of blood microcirculation during functional testing (occlusion, heat, cold, orthostatic) in different combinations, duration of action and created survey algorithm (Figure 1). Study included 52 patients with type 1DM and 2DM. The second step was to assess the feasibility of applying the algorithm for the diagnosis of microcirculatory disorders in patients with diabetes. Prognostic significance of measured parameters was evaluated by using ROC-analysis. A logistic regression model was built. The study included 10 patients with type 2DM (HbA1c level - 8.9 +/- 1.2%), the duration of DM was more than 5 years. The control group consisted of 10 healthy volunteers. Blood microcirculation in skin was measured by using LAKK-02 system. Results: Results of registration of the skin perfusion are shown in the table 1. Ratio of median base perfusion to median perfusion during combined functional impact (relative perfusion index) allowed us to classify the surveyed persons by groups with or without microcirculation disorders with 80% sensitivity and specificity. Area under the ROC-curve (AUC) was 0.93, 95% confidence interval for the AUC was 0.81-1 (Figure 2). Conclusion: The developed method has demonstrated the efficacy of detection of microcirculatory disorders in patients with diabetes. Received results can considered as foundation for the wide-scale study with the achievement of 90% power. (Figure Presented).