The Bolton and Hunter Method for Radiolabeling Protein

  • Bailey G
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Abstract

This is an indirect method in which an acylating reagent (N-succinimidyl-3 [4-hydroxyphenyl]propionate, the Bolton and Hunter reagent), commercially available in a radioiodinated form, is covalently coupled to the protein to be labeled (1). The [125I] Bolton and...

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Bailey, G. S. (2009). The Bolton and Hunter Method for Radiolabeling Protein (pp. 1733–1734). https://doi.org/10.1007/978-1-59745-198-7_176

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