A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses (Variola, Monkeypox, and Ectromelia viruses) demonstrates a considerable increase in the fluorescence signal (up to 100-fold) when several oligonucleotide probes are printed to one spot. Moreover, the specificity of detection also increases (almost 1000-fold), allowing the use of probes that individually lack such high specificity. The optimal probes have a Tm of 32–37 °C and length of 13–15 bases. We suggest that the high specificity and sensitivity of the MPOS-microarray is a result of cooperativity of DNA binding with all probes immobilized in the spot. This variant of DNA detection can be useful for designing biosensors, tools for point-of-care (POC) diagnostics, microbial ecology, analysis of clustered regularly interspaced short palindromic repeats (CRISPR), and others. [Figure not available: see fulltext.].
CITATION STYLE
Kostina, E. V., Sinyakov, A. N., & Ryabinin, V. A. (2018). A many probes-one spot hybridization oligonucleotide microarray. Analytical and Bioanalytical Chemistry, 410(23), 5817–5823. https://doi.org/10.1007/s00216-018-1190-8
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