Waterborne diseases represent a significant public health risk worldwide and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control, and quality control data analysis. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Shanks, O. C., Peed, L., Sivaganesan, M., Haugland, R. A., & Chern, E. C. (2014). Human fecal source identification with real-time quantitative PCR. Methods in Molecular Biology, 1096, 85–99. https://doi.org/10.1007/978-1-62703-712-9_7
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