Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides. This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures. Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
CITATION STYLE
Liu, L. (2018). Methods for xyloglucan structure analysis in Brachypodium distachyon. In Methods in Molecular Biology (Vol. 1667, pp. 65–71). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7278-4_6
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