Detection and identification of citrus long-horned beetle, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) a new pest in Antalya Province, Turkey by sequencing of mtCOI region

Abstract

In 2016, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) was recorded for the first time on Acer negundo L. in Antalya Province, Turkey. Genetic identification of adult samples was made based on approximate alignment of a 729-bp sequence of the mitochondrial cytochrome oxidase I gene region. The sequence alignment in the BLAST analysis from the NCBI Genbank showed 99% similarity with A. chinensis. The sequence data was deposited to NCBI Genbank. A phylogenetic analysis was performed by using the sequence data belonging to A. chinensis and some species of same genus obtained from the NCBI. According to the phylogenetic analysis, populations of A. chinensis from Antalya and China were placed in the same subgroup and therefore it is postulated that the origin of the Antalya population is China.