Sequence approach of Elaeis guineensis for early detection of Ganoderma boninense resistance

Abstract

This study purposed to describe the product of Polymerase Chain Reaction (PCR) and sequencing of DNA from Elaeis guineensis root for Ganoderma boninense resistance in PT Socfin Indonesia. Based on PCR amplification a total of 18 sampling were detected, the percentage of positive results for G. boninense resistance was only 20%. The degree of sequence identity between DNA sequence known to be verified by the Blastx probability identity, E-value and total score. Only 11A genes that are available in GenBank and 15A and 16A gene found no similarity in the database of NCBI, therefore considered as new genes for probably G. boninense tolerance or resistance. The 11A gene showed the highest identity (98%) to predicted metallothionein like-protein with E-value 3e-19 and total scored 104. Metallothionein protein actives role in oil palm plants for resistance to G. boninense.