We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
CITATION STYLE
Guglielmi, L., & Martineau, P. (2009). Intrabody expression in eukaryotic cells. Methods in Molecular Biology (Clifton, N.J.), 562, 195–203. https://doi.org/10.1007/978-1-60327-302-2_15
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